The fruit fly, Drosophila melanogaster, was chosen due to its small size, short life cycle, significant genetic variability, and relatively inexpensive to propagate. Little is known about the effects of temperature on the development of fruit fly mutants, including scarlet eye, vestigial wing, white eye, yellow body, and wild-type strains. This study aimed to explore the effects on dry weight and body length among those five strains at two different temperatures, 20°C and 25°C. The data was analyzed using the t-test, ANOVA, and Tukey HSD test. The observation records showed that temperature did affect the number of days in each growth period for all five strains. The high temperature (25°C) shortened those periods. The low temperature (20°C) prolonged those periods. The ANOVA and Tukey DSH test found the difference between the dry weight and body length pairs among those five strains at 20ºC and 25ºC. The t-test results demonstrate the difference in dry weight and body length between the two temperature settings in females and males of each strain.